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HAYATI Journal of Biosciences

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Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

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Articles 9 Documents

Search results for , issue "Vol. 17 No. 3 (2010): September 2010" : 9 Documents clear

The Effect of Ascorbic Acid Treatment on Viability and Vigor Maize (Zea mays L.) Seedling under Drought Stress HAMIDAH HAMAMA; ENDANG MURNIATI
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

This study was conducted in the laboratory and the field to examine the effects of ascorbic acid treatment on germination and seedling growth under drought stress. The laboratory works consisted of two experiments and were designed to determine the critical osmotic potential of maize and to determine the optimum ascorbic acid concentration. The field study was designed to examine the effects of soaking seed in ascorbic acid on seedling growth under drought stress. Drought condition was simulated by PEG-6000 and regulation of water treatment. During the first experiment, interactions of both osmotic potential and varieties were significant at all variables. Germination percentage and speed of germination were significantly decreased by increasing of osmotic potential. The second experiment showed that interactions of both factors were significant at all variables except vigor index, the length of shoot, primary, and seminal root. The results showed that the ascorbic acid treatment improved the germination percentage, the speed of germination and the vigor index compared with the control, besides the increase in length of shoot, primary and seminal root and number of seminal root. However, the best result was showed by 55 mM ascorbic acid. The result of field experiment showed that interactions were not always significant and 55 mM ascorbic acid treatment increased the seedling height, the number of leaves and leaf area but it had no effect on the water deficit and the root length.

Nursery Culture Performance of Litopenaeus vannamei with Probiotics Addition and Different C/N Ratio Under Laboratory Condition . WIDANARNI; DEBY YUNIASARI; . SUKENDA; JULIE EKASARI
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

Application of bioflocs technology and probiotics has improved water quality and production of Pacific white shrimp (Litopenaeus vannamei) culture. This experiment was to verify the effect of probiotic bacteria addition and different carbon:nitrogen (C:N) ratio on water quality and performance of Pacific white shrimp nursery culture. Nursery culture was carried out for 25 days in an aquarium under laboratory condition with stock density of one Post-Larvae (PL) (poslarval) per liter (24 PL/aquarium) of PL16 shrimp. Different C:N ratio resulted a significant difference on shrimp production performance. Treatment of 10 C:N ratio demonstrated the best shrimp growth (20.37 + 0.48% per day in weight and 6.05 + 0.41% per day in length), harvesting yield (1180 + 62 g/m3) and feed efficiency (121 + 6%). There was however no significant difference observed between treatments in water quality.

Soy Germed Protein Plus Zn as an Inducer Insulin Secretion on Type-2 Diabetes Mellitus HERY WINARSI; AGUS PURWANTO
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

Hyperglicemic induces pancreatic cells to produce inadequate insulin. However, previous studies revealed that soy protein induce pancreatic cells to secrete insulin. Hence, this study was aimed to investigate effect of soy germed protein on the insulin and blood glucose level of type-2 diabetes mellitus with Zn enrichment. The research involved twenty four women that characterized with having more blood glucose level than normal, body mass index more than twenty three kg/m2, and age more than fourty years old. They were divided into three groups randomly, eight woman for each group. The first, second and third group were treated respectively with milk containing soy germed protein plus Zn, this product without Zn, and placebo, all for two months. Blood samples were taken at baseline, one and two months after observation. Results showed that two months after observation the insulin level increased from 194.79 to 519.82 pmol/ml (P = 0.033) in group consuming milk containing soy germed protein with or without Zn, supported by significantly reduced blood glucose level. This result might be correlated with the potency of isoflavones in soy germ protein to protect pancreatic beta cellsmembrane from free radicals attack. Therefore, this maintain the cells integrity and to secrete optimal insulin.

Primary Nutritional Content of Bio-Flocs Cultured with Different Organic Carbon Sources and Salinity JULIE EKASARI; ROSELIEN CRAB; WILLY VERSTRAETE
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

Application of bio-flocs technology (BFT) in aquaculture offers a solution to avoid environmental impact of high nutrient discharges and to reduce the use of artificial feed. In BFT, excess of nutrients in aquaculture systems are converted into microbial biomass, which can be consumed by the cultured animals as a food source. In this experiment, upconcentrated pond water obtained from the drum filter of a freshwater tilapia farm was used for bio-flocs reactors. Two carbon sources, sugar and glycerol, were used as the first variable, and two different levels of salinity, 0 and 30 ppt, were used as the second variable. Bio-flocs with glycerol as a carbon source had higher total n-6 PUFAs (19.1 + 2.1 and 22.3 + 8.6 mg/g DW at 0 and 30 ppt, respectively) than that of glucose (4.0 + 0.1 and 12.6 + 2.5 mg/g DW at 0 and 30 ppt). However, there was no effect of carbon source or salinity on crude protein, lipid, and total n-3 PUFAs contents of the bio-flocs.

Root and Nodulation Phenotypes of the Ethylene-Insensitive Sickle Mutant of Medicago truncatula JOKO PRAYITNO
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

The sickle (skl) mutant of the model legume Medicago truncatula is an ethylene-sensitive mutant that have a ten-fold increase in nodule numbers. The nodulation and root phenotypes of the skl mutant were investigated and further characterised. The skl mutant had longer roots than the wild type, but when inoculated with Sinorhizobium, its root length was reduced to the level of wild type. Furthermore, lateral root numbers in uninoculated skl were similar to those in uninoculated wild type. However, when the root tips were decapitated, fewer lateral roots formed in skl than in wild type. Nodule numbers of the skl mutant were significantly reduced by low nitrate concentration (2.5 mM). These results suggest that skl mutant has alterations in both root and nodule development.

Population Status and Ecological Preferences of the Palm Sommieria leucophylla Beccari in Salawati Island DIDIK WIDYATMOKO
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

Population status and ecological preferences of the New Guinean endemic palm Sommieria leucophylla in a lowland forest of the North Salawati Island Nature Reserve were documented at six different habitat types: river bank, hill slope, hill top, intact, disturbed, and converted forests. Population sizes varied spatially and were dominated by seedlings and juveniles, indicating a growing population. Individuals with stem heights of 0-100 cm and stem diameters of 2-3 cm dominated. The stem height class distribution showed a preponderance of individuals in the juvenile stage class and a strong right hand skew typical of populations in which recruitment and mortality were continuous and density dependent, rather than episodic. S. leucopylla preferred specific habitats with river bank and intact forest being the most suitable habitat. Although the palm tolerated hill slopes, the populations were low and even suppressed at hill tops, and seemed to be sensitive to disturbance and changes in water table. Mortality was higher among the early stages but very low in adults. There was little recruitment in disturbed sites and no establishment in converted forests. To conserve the most important remaining populations, it is crucial to protect the most suitable sites in the reserve.

Diversity of Antifungal Compounds-Producing Bacillus spp. Isolated from Rhizosphere of Soybean Plant Based on ARDRA and 16S rRNA ARIS TRI WAHYUDI; BRAMANTYO JATI PRASOJO; NISA RACHMANIA MUBARIK
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

Plant growth promoting rhizobacteria (PGPR) play an important role in improvement of seed germination, root development, and water utilization by plants. These rhizobacteria can stimulate plant growth directly by producing growth hormones or indirectly by producing antifungal compounds/antibiotics to suppress phytopathogenic fungi. The objective of this research was to analyze the diversity of 22 antifungal-producing rhizobacteria of Bacillus sp. isolated from rhizosphere of soybean plant based on Amplified rDNA Restriction Analysis (ARDRA) and 16S rRNA Sequence. Restriction enzymes in ARDRA analysis, HinfI, HaeIII, and RsaI were used to digest 22 16S rDNA amplified from Bacillus sp. genomes. Based on this analysis, genetic diversity of 22 Bacillus sp. producing antifungal compounds were classified into eight different groups. Moreover, six selected isolates randomly from each ARDRA group that have strong activity to suppress fungal growth were analyzed for their 16S rDNA sequences compared with reference strains. The distributions of these isolates were genetically diverse on several species of Bacillus sp. such as B. subtilis, B. cereus, and B. fusiformis. ARDRA is a reliable technique to analyze genetic diversity of Bacillus sp. community in the rhizosphere.

Bioenergetic Consequences of FLAG Tag Addition to the C-Terminus of Subunit 8 of Yeast Saccharomyces cerevisiae Mitochondrial ATP Synthase I MADE ARTIKA
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

The yeast mitochondrial F1F0-ATP synthase is a multisubunit complex that contains at least 17 different subunits. Subunit 8 of yeast mitochondrial ATP synthase is a hydrophobic protein of 48 amino acids encoded by the mitochondrial ATP8 gene. Subunit 8 has three distinct domains; an N-terminal domain, a central hydrophobic domain and a C-terminal domain. FLAG tag addition to subunit 8 protein potentially facilitate elucidation of its topology, structure, and function. It has been shown that following incorporation of FLAG tag to its C-terminus, subunit 8 still assemble into functional ATP synthase complex. In order to analyze bioenergetic consequences of the FLAG tag addition, a yeast strain expressing FLAG tagged-subunit 8 was subjected to cellular respiration assays. Results obtained showed that addition of FLAG tag to the C-terminus of subunit 8 does not impair its proper functioning. The FLAG tag system, therefore, can be employed to study subunit 8’s detailed structure, topology, and function.

Myoglobin Expression in Chelonia mydas Brain, Heart and Liver Tissues RINI PUSPITANINGRUM; SEPTELIA INAWATI WANANDI; RONDANG ROEMIATI SOEGIANTO; MOHAMAD SADIKIN; DARYL ROBERT WILLIAMS; ANDREW ROBERT COSSINS
HAYATI Journal of Biosciences Vol. 17 No. 3 (2010): September 2010
Publisher : Bogor Agricultural University, Indonesia

An understanding of the underpinning physiology and biochemistry of animals is essential to properly understand the impact of anthropogenic changes and natural catastrophes upon the conservation of endangered species. An observation on the tissue location of the key respiratory protein, myoglobin, now opens up new opportunities for understanding how hypoxia tolerance impacts on diving lifestyle in turtles. The respiratory protein, myoglobin has functions other than oxygen binding which are involved in hypoxia tolerance, including metabolism of reactive oxygen species and of the vascular function by metabolism of nitric oxide. Our work aims to determine whether myoglobin expression in the green turtle exists in multiple non muscle tissues and to confirm the hypothesis that reptiles also have a distributed myoglobin expression which is linked to the hypoxiatolerant trait. This initial work in turtle hatch Chelonia mydas confirms the presence of myoglobin transcriptin brain, heart and liver tissues. Furthermore, it will serve as a tool for completing the sequence and generating an in situ hybridization probe for verifying of cell location in expressing tissues.

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